Expression of lycopene cyclase genes and their regulation on downstream carotenoids during fruit maturation of Guoqing No. 1 Satsuma mandarin and Cara Cara navel orange
Identifieur interne : 001950 ( Main/Exploration ); précédent : 001949; suivant : 001951Expression of lycopene cyclase genes and their regulation on downstream carotenoids during fruit maturation of Guoqing No. 1 Satsuma mandarin and Cara Cara navel orange
Auteurs : YONG WANG [République populaire de Chine] ; JING LI [République populaire de Chine] ; JING YANG [République populaire de Chine] ; RENXUE XIA [République populaire de Chine]Source :
- Scientia horticulturae [ 0304-4238 ] ; 2011.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Fruit, Horticulture, Enzyme.
English descriptors
- KwdEn :
Abstract
Lycopene β-cyclase (LCYb) and lycopene ε-cyclase (LCYe) are believed to be crucial genes to lycopene cyclization and downstream carotenoid accumulation. To illuminate the gene expression profiles and their regulation on downstream carotenoids, expression profiles of these two genes and contents of several corresponding carotenoids were determined by real-time PCR and HPLC, respectively, in relative shorter intervals during breaker and maturation stage of Guoqing No. 1 satsuma mandarin (Citrus unshiu Marcow) and Cara Cara navel orange (Citrus sinensis Osbeck). Data showed that the expression profiles of these two cyclase genes and their relative expression levels between the peels and the pulps at the same stage all varied with cultivars. But in general, accumulation oflutein and β-carotene experienced a similar tendency in both cultivars and were up-regulated by expression of LCYb and LCYe in spite of poor correlation in the pulps. Meanwhile, lycopene abundance was negatively correlated with the expression of LCYe as expected, but hardly correlated with LCYb in the pulps of Cara Cara navel orange, indicating that LCYe might exert a more prominent influence on lycopene cyclization and downstream carotenoids formation. Results ofcarotenoids accumulation suggest that the biosynthetic process of carotenoids would shift from upstream β, ε- or β, β-carotenoids such as lutein and β-carotene to downstream β, β-carotenoids during fruit development.
Affiliations:
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lycopene cyclase genes and their regulation on downstream carotenoids during fruit maturation of Guoqing No. 1 Satsuma mandarin and Cara Cara navel orange</title><author><name sortKey="Yong Wang" sort="Yong Wang" uniqKey="Yong Wang" last="Yong Wang">YONG WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University</s1><s2>Wuhan 430070</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author><author><name sortKey="Jing Li" sort="Jing Li" uniqKey="Jing Li" last="Jing Li">JING LI</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>The Subject Group of the Molecular Breeding of Fruit Trees, Wuhan Botanical Garden, Chinese Academy of Sciences</s1><s2>Wuhan 430070</s2><s3>CHN</s3><sZ>2 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author><author><name sortKey="Jing Yang" sort="Jing Yang" uniqKey="Jing Yang" last="Jing Yang">JING YANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University</s1><s2>Wuhan 430070</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author><author><name sortKey="Renxue Xia" sort="Renxue Xia" uniqKey="Renxue Xia" last="Renxue Xia">RENXUE XIA</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University</s1><s2>Wuhan 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time</term><term>Ripening</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Gène</term><term>Fruit</term><term>Maturation</term><term>Réaction chaîne polymérase</term><term>Temps réel</term><term>Chromatographie HPLC</term><term>Horticulture</term><term>Citrus unshiu</term><term>Citrus sinensis</term><term>Lycopène</term><term>Enzyme</term><term>Caroténoïde</term><term>Orange</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Fruit</term><term>Horticulture</term><term>Enzyme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lycopene β-cyclase (LCYb) and lycopene ε-cyclase (LCYe) are believed to be crucial genes to lycopene cyclization and downstream carotenoid accumulation. To illuminate the gene expression profiles and their regulation on downstream carotenoids, expression profiles of these two genes and contents of several corresponding carotenoids were determined by real-time PCR and HPLC, respectively, in relative shorter intervals during breaker and maturation stage of Guoqing No. 1 satsuma mandarin (Citrus unshiu Marcow) and Cara Cara navel orange (Citrus sinensis Osbeck). Data showed that the expression profiles of these two cyclase genes and their relative expression levels between the peels and the pulps at the same stage all varied with cultivars. But in general, accumulation oflutein and β-carotene experienced a similar tendency in both cultivars and were up-regulated by expression of LCYb and LCYe in spite of poor correlation in the pulps. Meanwhile, lycopene abundance was negatively correlated with the expression of LCYe as expected, but hardly correlated with LCYb in the pulps of Cara Cara navel orange, indicating that LCYe might exert a more prominent influence on lycopene cyclization and downstream carotenoids formation. Results ofcarotenoids accumulation suggest that the biosynthetic process of carotenoids would shift from upstream β, ε- or β, β-carotenoids such as lutein and β-carotene to downstream β, β-carotenoids during fruit development.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Yong Wang" sort="Yong Wang" uniqKey="Yong Wang" last="Yong Wang">YONG WANG</name></noRegion><name sortKey="Jing Li" sort="Jing Li" uniqKey="Jing Li" last="Jing Li">JING LI</name><name sortKey="Jing Yang" sort="Jing Yang" uniqKey="Jing Yang" last="Jing Yang">JING YANG</name><name sortKey="Renxue Xia" sort="Renxue Xia" uniqKey="Renxue Xia" last="Renxue Xia">RENXUE XIA</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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